CaMKinet | Result

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Sequence Viewer

Parameter	Value
Gene	GBF1
Protein Name	GBF1_HUMAN
Organism	Homo sapiens (Human)
Alternative name(s)	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (BFA-resistant GEF 1)
Protein Family	N/A
NCBI Gene ID	8729
UniProt ID	Q92538
Enzyme Class	-
Molecular Weight	206446
Protein Length	1859
Protein Domain	InterPro \| Pfam
3D Structure	PDBe \| PDBj \| RCSB PDB \| DrugPort ModBase \| SwissModel
Gene Expression	Gene Expression Atlas
Function and Disease	OMIM
Protein-protein Interaction Database	STRING \| IntAct \| MINT
Kinase Database	Phospho.ELM \| PhosphoSite \| NetworKIN
Catalytic Activity (UniProt annotation)	-
Localization	Golgi apparatus, cis-Golgi network
Function (UniProt annotation)	Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5 (By similarity). Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891).
Gene Ontology	GO:0000139; GO:0002263; GO:0005085; GO:0005086; GO:0005547; GO:0005739; GO:0005777; GO:0005788; GO:0005793; GO:0005794; GO:0005795; GO:0005801; GO:0005802; GO:0005811; GO:0005829; GO:0006888; GO:0006890; GO:0006892; GO:0006895; GO:0007030; GO:0007346; GO:0015031; GO:0016020; GO:0016032; GO:0030593; GO:0031252; GO:0032012; GO:0034067; GO:0042147; GO:0048205; GO:0061162; GO:0070973; GO:0080025; GO:0090166; GO:0097111; GO:0098586; GO:1903409; GO:1903420; GO:2000008

Gene Ontology

Gene Name	Organism	P-Site	Sequence(+/-7)	Conservation	Disorder	Curator Assessment	Reliability	Evidence Class	Evidence Logic	PubMed	Phospho-ELM	PhosphoSite-Plus
PRKAA1 (Q13131)	Homo sapiens	T1337	GKIHRSATDADVVNS	0.284	0.4582	TP	certain	experimental	73 \| 55 \| 9 \| 20 \| 72 \|	18063581	-	-

Interaction Network

Reactome Pathways

Pathway ID	Pathway Name
R-HSA-1280215	Cytokine Signaling in Immune system \| Reactome Pathway
R-HSA-168256	Immune System \| Reactome Pathway
R-HSA-449147	Signaling by Interleukins \| Reactome Pathway
R-HSA-6785807	Interleukin-4 and Interleukin-13 signaling \| Reactome Pathway

No KEGG pathways found

NCI Nature PID Pathways

Pathway ID	Pathway Name
arf_3pathway	Arf1 pathway