STRING | IntAct | MINT

Open Targets | OMIM

Catalytic Site Atlas

ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]

Cytoplasm,Cell membrane,Mitochondrion,Nucleus;

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

GO:0005737; GO:0005829; GO:0005794; GO:0005741; GO:0005739; GO:0016607; GO:0005886; GO:0031143; GO:0010856; GO:0008179; GO:0005524; GO:0019899; GO:0042802; GO:0016301; GO:0004709; GO:0046872; GO:0031434; GO:0046982; GO:0004672; GO:0004674; GO:0007190; GO:0000186; GO:0006915; GO:0030154; GO:0008283; GO:0071550; GO:0060324; GO:0007507; GO:0035773; GO:0045104; GO:0034220; GO:0000165; GO:0043066; GO:0008285; GO:0043154; GO:1902042; GO:0031333; GO:0048011; GO:0030168; GO:0033138; GO:0045944; GO:0006468; GO:0042981; GO:0045595; GO:2000145; GO:0035023; GO:0001666; GO:0035994; GO:0007165; GO:0035019; GO:0002223; GO:0048538; GO:0030878; GO:0042060