CaMKinet | Result

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Sequence Viewer

Parameter	Value
Gene	NOS3
Protein Name	NOS3_HUMAN
Organism	Homo sapiens (Human)
Alternative name(s)	Nitric oxide synthase, endothelial (EC 1.14.13.39) (Constitutive NOS) (cNOS) (EC-NOS) (Endothelial NOS) (eNOS) (NOS type III) (NOSIII)
Protein Family	NOS family
NCBI Gene ID	4846
UniProt ID	P29474
Enzyme Class	1.14.13.39
Molecular Weight	133275
Protein Length	1203
Protein Domain	InterPro \| Pfam
3D Structure	PDBe \| PDBj \| RCSB PDB \| DrugPort ModBase \| SwissModel
Gene Expression	Gene Expression Atlas
Function and Disease	OMIM
Protein-protein Interaction Database	STRING \| IntAct \| MINT
Kinase Database	Phospho.ELM \| PhosphoSite \| NetworKIN
Catalytic Activity (UniProt annotation)	2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
Localization	Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Function (UniProt annotation)	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.; Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Gene Ontology	GO:0000139; GO:0001525; GO:0001542; GO:0001701; GO:0001974; GO:0002028; GO:0003057; GO:0003100; GO:0003180; GO:0003184; GO:0003203; GO:0003785; GO:0003958; GO:0004517; GO:0005516; GO:0005634; GO:0005737; GO:0005794; GO:0005829; GO:0005856; GO:0005886; GO:0005901; GO:0006527; GO:0006809; GO:0007005; GO:0007263; GO:0008217; GO:0008285; GO:0009408; GO:0009725; GO:0010181; GO:0010544; GO:0010628; GO:0012506; GO:0014740; GO:0014806; GO:0016491; GO:0019430; GO:0020037; GO:0030324; GO:0030666; GO:0031284; GO:0031663; GO:0032496; GO:0034405; GO:0034617; GO:0034618; GO:0042311; GO:0043267; GO:0043536; GO:0043542; GO:0045454; GO:0045766; GO:0045776; GO:0046870; GO:0048873; GO:0050660; GO:0050661; GO:0050880; GO:0050999; GO:0051186; GO:0051346; GO:0051926; GO:0060412; GO:0070168; GO:0097110; GO:1902042

Gene Ontology

Gene Name	Organism	P-Site	Sequence(+/-7)	Conservation	Disorder	Curator Assessment	Reliability	Evidence Class	Evidence Logic	PubMed	Phospho-ELM	PhosphoSite-Plus
PRKAA1 (Q13131)	Homo sapiens	S1177	TSRIRTQSFSLQERQ	0.14	0.4409	TP	certain	experimental	72 \| 55 \|	16126727	-	In vivo
Prkaa2 (Q09137)	Rattus norvegicus	S1177	TSRIRTQSFSLQERQ	0.14	0.4409	TP	certain	experimental	30 \| 55 \| 23 \| 135 \|	10025949	-	-
Prkaa1 (P54645)	Rattus norvegicus	S1177	TSRIRTQSFSLQERQ	0.14	0.4409	TP	certain	experimental	30 \| 55 \| 23 \| 135 \|	10025949	-	-
Prkaa2 (Q09137)	Rattus norvegicus	T495	TGITRKKTFKEVANA	0.231	0.3087	TP	certain	experimental	30 \| 55 \| 23 \| 135 \|	10025949	-	-
Prkaa1 (P54645)	Rattus norvegicus	T495	TGITRKKTFKEVANA	0.231	0.3087	TP	certain	experimental	30 \| 55 \| 23 \| 135 \|	10025949	-	-
PRKAA1 (Q13131)	Homo sapiens	S633	WRRKRKESSNTDSAG	0.163	0.4801	-	-	-	-	-	-	In vivo
PRKAA2 (P54646)	Homo sapiens	S1177	TSRIRTQSFSLQERQ	0.14	0.4409	-	-	-	-	-	-	-
PRKAA2 (P54646)	Homo sapiens	S633	WRRKRKESSNTDSAG	0.163	0.4801	-	-	-	-	-	-	-

Interaction Network

Reactome Pathways

Pathway ID	Pathway Name
R-HSA-1280215	Cytokine Signaling in Immune system \| Reactome Pathway
R-HSA-168256	Immune System \| Reactome Pathway
R-HSA-449147	Signaling by Interleukins \| Reactome Pathway
R-HSA-6785807	Interleukin-4 and Interleukin-13 signaling \| Reactome Pathway

KEGG Pathways

Pathway ID	Pathway Name
hsa00330	Arginine and proline metabolism
hsa01100	Metabolic pathways
hsa04020	Calcium signaling pathway
hsa04370	VEGF signaling pathway

NCI Nature PID Pathways

Pathway ID	Pathway Name
angiopoietinreceptor_pathway	Angiopoietin receptor Tie2-mediated signaling
er_nongenomic_pathway	Plasma membrane estrogen receptor signaling
txa2pathway	Thromboxane A2 receptor signaling
vegfr1_2_pathway	Signaling events mediated by VEGFR1 and VEGFR2
vegfr1_pathway	VEGFR1 specific signals