CaMKinet | Result

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Sequence Viewer

Parameter	Value
Gene	MDM2
Protein Name	MDM2_HUMAN
Organism	Homo sapiens (Human)
Alternative name(s)	E3 ubiquitin-protein ligase Mdm2 (EC 2.3.2.27) (Double minute 2 protein) (Hdm2) (Oncoprotein Mdm2) (RING-type E3 ubiquitin transferase Mdm2) (p53-binding protein Mdm2)
Protein Family	MDM2/MDM4 family
NCBI Gene ID	4193
UniProt ID	Q00987
Enzyme Class	2.3.2.27
Molecular Weight	55233
Protein Length	491
Protein Domain	InterPro \| Pfam
3D Structure	PDBe \| PDBj \| RCSB PDB \| DrugPort ModBase \| SwissModel
Gene Expression	Gene Expression Atlas
Function and Disease	OMIM
Protein-protein Interaction Database	STRING \| IntAct \| MINT
Kinase Database	Phospho.ELM \| PhosphoSite \| NetworKIN
Catalytic Activity (UniProt annotation)	S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
Localization	Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
Function (UniProt annotation)	E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity).
Gene Ontology	GO:0000122; GO:0001568; GO:0001974; GO:0002027; GO:0002039; GO:0003181; GO:0003203; GO:0003281; GO:0003283; GO:0004842; GO:0005634; GO:0005654; GO:0005730; GO:0005737; GO:0005829; GO:0005886; GO:0006511; GO:0006977; GO:0007089; GO:0008097; GO:0008270; GO:0008284; GO:0010039; GO:0010628; GO:0010955; GO:0010977; GO:0016032; GO:0016567; GO:0016579; GO:0016874; GO:0018205; GO:0019789; GO:0019899; GO:0019904; GO:0030666; GO:0031625; GO:0031648; GO:0032026; GO:0032436; GO:0032991; GO:0033612; GO:0034504; GO:0036369; GO:0042176; GO:0042220; GO:0042802; GO:0042975; GO:0043021; GO:0043130; GO:0043154; GO:0043278; GO:0043518; GO:0045184; GO:0045202; GO:0045472; GO:0045892; GO:0045931; GO:0046677; GO:0046827; GO:0047485; GO:0048545; GO:0051603; GO:0051865; GO:0060411; GO:0061630; GO:0061663; GO:0065003; GO:0070301; GO:0071157; GO:0071301; GO:0071312; GO:0071363; GO:0071375; GO:0071391; GO:0071456; GO:0071480; GO:0071494; GO:0072717; GO:0097110; GO:0097718; GO:1901796; GO:1901797; GO:1902254; GO:1904404; GO:1904707; GO:1904754; GO:1990000; GO:1990785

Gene Ontology

Gene Name	Organism	P-Site	Sequence(+/-7)	Conservation	Disorder	Curator Assessment	Reliability	Evidence Class	Evidence Logic	PubMed	Phospho-ELM	PhosphoSite-Plus
MAPKAPK2 (P49137)	Homo sapiens	S157	SHLVSRPSTSSRRRA	0.615	0.6482	TP	certain	experimental	72 \| 73 \| 55 \| 9 \| 273 \|	15688025	-	In vitro
MAPKAPK2 (P49137)	Homo sapiens	S166	SSRRRAISETEENSD	0.815	0.7799	TP	certain	experimental	72 \| 73 \| 55 \| 9 \| 273 \|	15688025	-	In vitro
PIM1 (P11309)	Homo sapiens	S166	SSRRRAISETEENSD	0.815	0.7799	TP	certain	experimental	72 \| 73 \| 8 \| 55 \| 107 \| 23 \|	18467333	-	In vitro and in vivo
PIM1* (P11309)	Homo sapiens	S186	RQRKRHKSDSISLSF	0.958	0.6136	TP	likely	experimental	73 \| 8 \| 55 \| 107 \| 72 \| 23 \|	18467333	-	In vitro and in vivo
DAPK1 (P53355)	Homo sapiens	S166	SSRRRAISETEENSD	0.815	0.7799	TP	certain	-	161 \| 131 \| 9 \| 73 \|	15001356	-	-
DAPK1 (P53355)	Homo sapiens	S186	RQRKRHKSDSISLSF	0.958	0.6136	TP	certain	-	161 \| 131 \| 9 \| 73 \|	15001356	-	-
DAPK3 (O43293)	Homo sapiens	S186	RQRKRHKSDSISLSF	0.958	0.6136	-	-	-	-	-	-	In vitro
RPS6KA1 (Q15418)	Homo sapiens	S166	SSRRRAISETEENSD	0.815	0.7799	-	-	-	-	-	-	-
RPS6KA1 (Q15418)	Homo sapiens	S186	RQRKRHKSDSISLSF	0.958	0.6136	-	-	-	-	-	-	-
PIM2 (Q9P1W9)	Homo sapiens	S166	SSRRRAISETEENSD	0.815	0.7799	-	-	-	-	-	-	-
PIM2 (Q9P1W9)	Homo sapiens	S186	RQRKRHKSDSISLSF	0.958	0.6136	-	-	-	-	-	-	-

Interaction Network

Reactome Pathways

Pathway ID	Pathway Name
R-HSA-1280215	Cytokine Signaling in Immune system \| Reactome Pathway
R-HSA-168256	Immune System \| Reactome Pathway
R-HSA-449147	Signaling by Interleukins \| Reactome Pathway
R-HSA-6785807	Interleukin-4 and Interleukin-13 signaling \| Reactome Pathway

KEGG Pathways

Pathway ID	Pathway Name
hsa04110	Cell cycle
hsa04115	p53 signaling pathway
hsa04120	Ubiquitin mediated proteolysis
hsa04144	Endocytosis
hsa05200	Pathways in cancer
hsa05214	Glioma
hsa05215	Prostate cancer
hsa05218	Melanoma
hsa05219	Bladder cancer
hsa05220	Chronic myeloid leukemia

NCI Nature PID Pathways

Pathway ID	Pathway Name
ar_tf_pathway	Regulation of Androgen receptor activity
aurora_a_pathway	Aurora A signaling
pi3kciaktpathway	Class I PI3K signaling events mediated by Akt
ranbp2pathway	Sumoylation by RanBP2 regulates transcriptional repression