CaMKinet | Result

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Sequence Viewer

Parameter	Value
Gene	PRKAA1
Protein Name	AAPK1_HUMAN
Organism	Homo sapiens (Human)
Alternative name(s)	5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1) (EC 2.7.11.1) (Acetyl-CoA carboxylase kinase) (ACACA kinase) (EC 2.7.11.27) (Hydroxymethylglutaryl-CoA reductase kinase) (HMGCR kinase) (EC 2.7.11.31) (Tau-protein kinase PRKAA1) (EC 2.7.11.26)
Protein Family	Protein kinase superfamily
NCBI Gene ID	5562
UniProt ID	Q13131
Enzyme Class	2.7.11.1; 2.7.11.27; 2.7.11.31; 2.7.11.26
Molecular Weight	64009
Protein Length	559
Protein Domain	InterPro \| Pfam
3D Structure	PDBe \| PDBj \| RCSB PDB \| DrugPort ModBase \| SwissModel
Gene Expression	Gene Expression Atlas
Function and Disease	OMIM
Protein-protein Interaction Database	STRING \| IntAct \| MINT
Kinase Database	Phospho.ELM \| PhosphoSite \| NetworKIN
Catalytic Activity (UniProt annotation)	ATP + a protein = ADP + a phosphoprotein.
Localization	Cytoplasm Note=In response to stress, recruited by p53/TP53 to specific promoters.
Function (UniProt annotation)	Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
Gene Ontology	GO:0000187; GO:0001666; GO:0003682; GO:0004672; GO:0004674; GO:0004679; GO:0004691; GO:0005524; GO:0005634; GO:0005654; GO:0005737; GO:0005829; GO:0006006; GO:0006468; GO:0006633; GO:0006695; GO:0007050; GO:0007165; GO:0008022; GO:0008284; GO:0008610; GO:0009411; GO:0009631; GO:0010332; GO:0010508; GO:0010628; GO:0010629; GO:0014823; GO:0016055; GO:0016236; GO:0016324; GO:0016607; GO:0019395; GO:0030424; GO:0030425; GO:0031000; GO:0031588; GO:0031669; GO:0032007; GO:0033135; GO:0034599; GO:0035174; GO:0035556; GO:0042149; GO:0042593; GO:0042752; GO:0043025; GO:0043066; GO:0045542; GO:0045821; GO:0046318; GO:0046872; GO:0047322; GO:0048511; GO:0048643; GO:0050321; GO:0050405; GO:0050995; GO:0051291; GO:0055089; GO:0060627; GO:0061744; GO:0061762; GO:0062028; GO:0070050; GO:0070301; GO:0070507; GO:0071277; GO:0071333; GO:0071361; GO:0071380; GO:0071417; GO:0071456; GO:0097009; GO:1901563; GO:1903109; GO:1903829; GO:1903955; GO:1904428; GO:2000758; GO:2001274

Gene Ontology

Gene Name	Organism	P-Site	Sequence(+/-7)	Conservation	Disorder	Curator Assessment	Reliability	Evidence Class	Evidence Logic	PubMed	Phospho-ELM	PhosphoSite-Plus
Prkaa1* (Q5EG47)	Mus musculus	S486	DEITEAKSGTATPQR	0.159	0.6991	TP	likely	experimental	73 \|	-	-	In vitro
PRKAA1 (Q13131)	Homo sapiens	S360	LATSPPDSFLDDHHL	0.163	0.3566	-	-	-	-	-	-	In vitro
PRKAA1 (Q13131)	Homo sapiens	S486	DEITEAKSGTATPQR	0.159	0.6991	-	-	-	-	-	-	In vitro
PRKAA1 (Q13131)	Homo sapiens	S494	GTATPQRSGSVSNYR	0.112	0.5854	-	-	-	-	-	-	In vitro
PRKAA1 (Q13131)	Homo sapiens	S496	ATPQRSGSVSNYRSC	0.23	0.6136	-	-	-	-	-	-	In vitro
PRKAA1 (Q13131)	Homo sapiens	T183	SDGEFLRTSCGSPNY	0.698	0.2951	-	-	-	-	-	-	In vitro
PRKAA1 (Q13131)	Homo sapiens	T388	ETPRARHTLDELNPQ	0.221	0.5665	-	-	-	-	-	-	In vitro
CAMK2B (Q13554)	Homo sapiens	T183	SDGEFLRTSCGSPNY	0.698	0.2951	-	-	-	-	-	-	In vitro
BRSK2 (Q8IWQ3)	Homo sapiens	T183	SDGEFLRTSCGSPNY	0.698	0.2951	-	-	-	-	-	-	In vitro
CAMKK1 (Q8N5S9)	Homo sapiens	T183	SDGEFLRTSCGSPNY	0.698	0.2951	-	-	-	-	-	-	In vivo
BRSK1 (Q8TDC3-2)	Homo sapiens	T183	SDGEFLRTSCGSPNY	0.698	0.2951	-	-	-	-	-	-	In vitro
CAMKK2 (Q96RR4)	Homo sapiens	T183	SDGEFLRTSCGSPNY	0.698	0.2951	-	-	-	-	-	-	In vivo
PRKD1 (Q15139)	Homo sapiens	S494	GTATPQRSGSVSNYR	0.112	0.5854	-	-	-	-	-	-	-
BRSK1 (Q8TDC3)	Homo sapiens	T183	SDGEFLRTSCGSPNY	0.698	0.2951	-	-	-	-	-	-	-

Interaction Network

Reactome Pathways

Pathway ID	Pathway Name
R-HSA-1280215	Cytokine Signaling in Immune system \| Reactome Pathway
R-HSA-168256	Immune System \| Reactome Pathway
R-HSA-449147	Signaling by Interleukins \| Reactome Pathway
R-HSA-6785807	Interleukin-4 and Interleukin-13 signaling \| Reactome Pathway

KEGG Pathways

Pathway ID	Pathway Name
hsa04140	Regulation of autophagy
hsa04150	mTOR signaling pathway
hsa04910	Insulin signaling pathway
hsa04920	Adipocytokine signaling pathway
hsa05410	Hypertrophic cardiomyopathy (HCM)

No NCI Nature pathways found